Journal: Signal Transduction and Targeted Therapy
Article Title: Glucocorticoids elevate clear cell renal cell carcinoma sensitivity to HIF-2α inhibitors by suppressing H4K12 lactylation
doi: 10.1038/s41392-026-02622-7
Figure Lengend Snippet: Six GCs inhibited H4K12la in ccRCC cells. a Experimental workflow of high-content drug screening for the quantification of H4K12la in 786-O control cells. 786-O cells were seeded in 384-well plates and then treated with FDA-approved drugs ( n = 2468) for 48 h. 786-O cells treated with vehicle were used as controls. Immunofluorescence staining of 786-O cells with H4K12la antibody (green). DAPI staining was used to visualize the nucleus (blue). High-content analysis with automatic image processing was applied to determine the mean immunofluorescence intensity per cell. b The relative average fluorescence intensity (RAFI) of 786-O cells treated with FDA-approved drugs compared with the vehicle control group. The drugs that caused a significant decrease in the relative average immunofluorescence intensity of H4K12la compared with that of the control group are labeled in red. A 40% reduction in the RAFI was considered to indicate a remarkable decrease (RAFI < 0.6, n = 15). c The top 15 drugs in terms of average fluorescence intensity compared with the vehicle control group. Blue, control group; red, GCs. d Immunofluorescence staining of 786-O cells treated with vehicle or the indicated GCs (10 μM), including DEX, BEC, FLUD, FLUO, MOME, and PRED, for 48 h with H4K12la antibody (green). DAPI staining was used to visualize the nucleus (blue). Bar, 50 μm. e Western blot analysis of H4K12la and H3K18la in 786-O cells treated with vehicle or the indicated drugs (10 μM) for 48 h. f Western blot analysis of H4K12la and H3K18la in 786-O cells treated with vehicle or DEX at the indicated concentrations for 48 h
Article Snippet: The agents used were belzutifan and SO (MedChemExpress, MCE), DEX, DCA, sodium lactate, and the FDA-approved drug library (Selleck).
Techniques: Drug discovery, Control, Immunofluorescence, Staining, High Content Screening, Fluorescence, Labeling, Western Blot
TargetMol is a verified supplier 
TargetMol manufactures this product 
